Total Starch Assay Kit (AA/AMG)
SKU: WZZZ2055
The Total Starch (AA/AMG) test kit is used for the measurement and analysis of total starch in cereal flours and food products. This kit now contains an improved α-amylase that allows the amylase incubations to be performed at pH 5.0 (as well as pH 7.0).
Advantages
• Very cost effective
• All reagents stable for > 12 years after preparation
• Simple format
• Mega-Calc™ software tool for hassle-free raw data processing
• Standard included
Principle:
(α-amylase, 100°C ± DMSO)
(1) Starch granules + H2O → maltodextrins
(amyloglucosidase)
(2) Maltodextrins + H2O → D-glucose
(glucose oxidase)
(3) D-Glucose + H2O + O2 → D-gluconate + H2O2
(peroxidase)
(4) 2H2O2 + p-hydroxybenzoic acid + 4-aminoantipyrine →
quinoneimine + 4H2O
Kit size: 50/100 assays
Method: Spectrophotometric at 510 nm
Total assay time: ~ 90 min
Detection limit: 1-100% of sample weight